en>NotWith: Category:Isoflavonoids metabolism

2013-07-07T07:02:10Z

Category:Isoflavonoids metabolism

← Older revision		Revision as of 09:02, 7 July 2013
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			\| Name = 3-hydroxybenzoate 4-monooxygenase
			\| EC_number = 1.14.13.23
			\| CAS_number = 37256-76-1
			\| IUBMB_EC_number = 1/14/13/23
			\| GO_code = 0018668
			\| image =
			\| width =
			\| caption =
			}}
			In [[enzymology]], a '''3-hydroxybenzoate 4-monooxygenase''' ({{EC number\|1.14.13.23}}) is an [[enzyme]] that [[catalysis\|catalyzes]] the [[chemical reaction]]

			:3-hydroxybenzoate + NADPH + H<sup>+</sup> + O<sub>2</sub> <math>\rightleftharpoons</math> 3,4-dihydroxybenzoate + NADP<sup>+</sup> + H<sub>2</sub>O

			The 4 [[substrate (biochemistry)\|substrates]] of this enzyme are [[3-hydroxybenzoate]], [[nicotinamide adenine dinucleotide phosphate\|NADPH]], [[hydrogen ion\|H<sup>+</sup>]], and [[oxygen\|O<sub>2</sub>]], whereas its 3 [[product (chemistry)\|products]] are [[3,4-dihydroxybenzoate]], [[nicotinamide adenine dinucleotide phosphate\|NADP<sup>+</sup>]], and [[water\|H<sub>2</sub>O]].

			This enzyme belongs to the family of [[oxidoreductase]]s, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of one atom o oxygen into the other donor. The systematic name of this enzyme class is '''3-hydroxybenzoate,NADPH:oxygen oxidoreductase (4-hydroxylating)'''. This enzyme is also called '''3-hydroxybenzoate 4-hydroxylase'''. This enzyme participates in [[benzoate degradation via hydroxylation]] and [[2,4-dichlorobenzoate degradation]]. It employs one [[cofactor (biochemistry)\|cofactor]], [[FAD]].

			==Structural studies==

			As of late 2007, two [[tertiary structure\|structures]] have been solved for this class of enzymes, with [[Protein Data Bank\|PDB]] accession codes {{PDB link\|2DKH}} and {{PDB link\|2DKI}}.

			==References==
			{{reflist\|1}}
			* {{cite journal \| author = Michalover JL, Ribbons DW, Hughes H \| date = 1973 \| title = 3-Hydroxybenzoate 4-hydroxylase from Pseudomonas testosteroni \| journal = Biochem. Biophys. Res. Commun. \| volume = 55 \| pages = 888–96 \| pmid = 4148586 \| doi = 10.1016/0006-291X(73)91227-8 \| issue = 3 }}
			* {{cite journal \| author = Premkumar R, Rao PV, Sreeleela NS, Vaidyanathan CS \| date = 1969 \| title = m-Hydroxybenzoic acid 4-hydroxylase from Aspergillus niger \| journal = Can. J. Biochem. \| volume = 47 \| pages = 825–7 \| pmid = 4390252 \| issue = 8 }}

			{{1.14.13-enzyme-stub}}

			[[Category:EC 1.14.13]]
			[[Category:NADPH-dependent enzymes]]
			[[Category:Flavin enzymes]]
			[[Category:Enzymes of known structure]]

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2-hydroxyisoflavanone synthase - Revision history

en>NotWith: Category:Isoflavonoids metabolism

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