https://en.formulasearchengine.com/w/api.php?action=feedcontributions&feedformat=atom&user=89.148.14.0%2F24formulasearchengine - User contributions [en]2026-09-24T14:34:31ZUser contributionsMediaWiki 1.47.0-wmf.7https://en.formulasearchengine.com/w/index.php?title=Overtime_rate&diff=17093Overtime rate2013-12-31T13:58:11Z<p>89.148.14.14: /* Calculation Formula */</p>
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<div>{{Pfam_box <br />
| Symbol = Urocanase <br />
| Name = Urocanase <br />
| image = PDB_2fkn_EBI.png<br />
| width = <br />
| caption = Crystal structure of Urocanase from ''B. subtilis''.<br />
| Pfam= PF01175<br />
| InterPro= IPR000193<br />
| SMART= <br />
| Prosite = PDOC00947<br />
| SCOP = <br />
| TCDB = <br />
| OPM family= <br />
| OPM protein= <br />
| PDB= <br />
{{PDB3|1uwk}}B:2-556 {{PDB3|1w1u}}A:2-556 {{PDB3|1uwl}}B:2-556 <br />
}} <br />
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[[Image:Urocanic acid.svg|thumb|[[Urocanic acid]]]]<br />
[[Image:Imidazol-4-one-5-propionic acid.png|thumb|[[Imidazol-4-one-5-propionic acid]]]]<br />
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'''Urocanase'''<ref name="PUB00000161">{{cite journal |author=Retey J |title=The urocanase story: a novel role of NAD+ as electrophile |journal=Arch. Biochem. Biophys. |volume=314 |issue=1 |pages=1–16 |year=1994 |pmid=7944380 |doi=10.1006/abbi.1994.1405}}</ref> (also known as '''imidazolonepropionate hydrolase''' or '''urocanate hydratase''') is the enzyme that catalyzes the second step in the degradation of histidine, the hydration of urocanate into imidazolonepropionate. <br />
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: [[urocanate]] + H<sub>2</sub>O <math>\rightleftharpoons</math> [[imidazol-4-one-5-propionic acid|4,5-dihydro-4-oxo-5-imidazolepropanoate]] <br />
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Inherited deficiency of urocanase leads to elevated levels of urocanic acid in the urine, a condition known as [[urocanic aciduria]].<br />
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Urocanase is found in some bacteria (gene hutU), in the liver of many vertebrates and has also been found in the plant ''[[Trifolium repens]]'' (white clover). Urocanase is a protein of about 60 Kd, it binds tightly to NAD<sup>+</sup> and uses it as an electrophil cofactor. A conserved cysteine has been found to be important for the catalytic mechanism and could be involved in the binding of the NAD<sup>+</sup>.<br />
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==References==<br />
{{reflist}}<br />
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==External links==<br />
* {{MeshName|Urocanate+Hydratase}}<br />
* {{EC number|4.2.1.49}}<br />
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{{Carbon-oxygen lyases}}<br />
{{Amino acid metabolism enzymes}}<br />
<br />
[[Category:Protein families]]<br />
[[Category:EC 4.2.1]]<br />
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{{Enzyme-stub}}</div>89.148.14.14