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| {{Evolutionary biology}}
| | However this isn't completely surprising, because a big waist (excess belly fat) is tied carefully to sort 2 diabetes, insulin resistance, a bad cholesterol profile plus heart condition. Excess fat in the belly indicates excess fat surrounding the abdominal organs -- never superior news; this form of fat is risky.<br><br>If left unchecked, plaque may build up in the arteries to the heart or mind leading to a heart attack or stroke. It's wise to keep an eye on your numbers.<br><br>Number 7. Test plus check a equipment before the marriage day. And by equipment, it literally means everything. From the camera, to the speed lights, a laptop, the reflectors plus naturally all of the batteries, check and test everything. Know which bulbs plus batteries have to be changed. There is really much space for error plus wedding photographers dont need their trusted equipment to fail on W-day, which might be a nightmare.<br><br>Since strokes are so dangerous, it's important to work on reducing the dangers that are under the control. Keep the blood pressure in a healthy range, as this is among the right elements you are able to do to fight the risk.<br><br>To discover out more, a research team searched health literature for research which looked at stroke risk and body mass index with a minimal of four years of follow up.<br><br>Underwater Weighing - Underwater weighing, or hydrostatic weighing, is another system to determine the body fat . In this procedure, a individual sits inside a specialized seat, expels the air from their lungs, and [http://safedietplansforwomen.com/waist-to-height-ratio waist to height ratio] is then submerged into a big steel tank of water to stay motionless while their underwater weight is recorded. This is repeated until a dependable fat is measured. The purpose is to measure density of the body plus calculate yourfat fat. Because lean tissue is denser than fat tissue, this system may determine the buoyancy of the person whilst underwater. Once considered the gold standard offat measurement, the equipment is quite costly, creating alternative techniques more appealing.<br><br>The techniques provided inside the program are based on the truth that there is not any fast means to burn excess body fat, however, it may nevertheless be possible by implementing the protocols, like following a diet plan with necessary exercises. The diet includes a list of food items including fruits that are capable to burn body fat immediately.<br><br>All info on this website is for informational reasons only. It is not intended to diagnose, treat, cure, or avoid any wellness problem - nor is it intended to replace the advice of a qualified healthcare specialist, health specialist, or fitness expert. No action could be taken only on the contents of this website. Always consult the doctor or qualified health specialist on any matters regarding your wellness or on any opinions expressed inside this site. |
| The '''molecular clock ''' (based on the '''molecular clock hypothesis''' ('''MCH''')) is a technique in [[molecular evolution]] that uses fossil constraints and rates of molecular change to deduce the time in [[Geologic time scale|geologic history]] when two [[species]] or other [[Taxon|taxa]] [[Genetic divergence|diverged]]. It is used to estimate the time of occurrence of events called [[speciation]] or [[Evolutionary radiation|radiation]]. The molecular data used for such calculations is usually [[nucleotide]] [[DNA sequence|sequences]] for [[DNA]] or [[amino acid]] sequences for [[protein]]s. It is sometimes called a '''gene clock''' or '''evolutionary clock'''.
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| ==Early discovery and genetic equidistance==
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| The notion of the existence of a so-called "molecular clock" was first attributed to [[Emile Zuckerkandl]] and [[Linus Pauling]] who, in 1962, noticed that the number of [[amino acid]] differences in [[hemoglobin]] between different lineages changes roughly [[Linear function|linearly]] with time, as estimated from fossil evidence.<ref name=Zuckerkand62>{{cite book | author = [[Emile Zuckerkandl|Zuckerkandl, E.]] and [[Linus Pauling|Pauling, L.B.]] | year = 1962 | title = Horizons in Biochemistry | chapter = Molecular disease, evolution, and genic heterogeneity | editor = Kasha, M. and Pullman, B (editors) | pages = 189–225 | publisher = Academic Press, New York}}</ref> They generalized this observation to assert that the rate of [[evolution]]ary change of any specified [[protein]] was approximately constant over time and over different lineages.
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| The '''genetic equidistance''' phenomenon was first noted in 1963 by E. Margoliash, who wrote: "It appears that the number of residue differences between [[cytochrome C]] of any two species is mostly conditioned by the time elapsed since the lines of evolution leading to these two species originally diverged. If this is correct, the cytochrome c of all mammals should be equally different from the cytochrome c of all birds. Since fish diverges from the main stem of vertebrate evolution earlier than either birds or mammals, the cytochrome c of both mammals and birds should be equally different from the cytochrome c of fish. Similarly, all vertebrate cytochrome c should be equally different from the yeast protein."<ref>{{cite journal |doi=10.1073/pnas.50.4.672 |author=Margoliash E |title=PRIMARY STRUCTURE AND EVOLUTION OF CYTOCHROME C |journal=Proc. Natl. Acad. Sci. U.S.A. |volume=50 |issue= 4|pages=672–9 |date=October 1963 |pmid=14077496 |pmc=221244}}</ref> For example, the difference between the cytochrome C of a carp and a frog, turtle, chicken, rabbit, and horse is a very constant 13% to 14%. Similarly, the difference between the cytochrome C of a bacterium and yeast, wheat, moth, tuna, pigeon, and horse ranges from 64% to 69%. Together with the work of Emile Zuckerkandl and Linus Pauling, the genetic equidistance result directly led to the formal postulation of the molecular clock hypothesis in the early 1960s.<ref>{{cite journal |author=Kumar S |title=Molecular clocks: four decades of evolution |journal=Nat. Rev. Genet. |volume=6 |issue=8 |pages=654–62 |date=August 2005 |pmid=16136655 |doi=10.1038/nrg1659}}</ref> Genetic equidistance has often been used to infer equal time of separation of different sister species from an outgroup.<ref>{{cite journal |doi=10.1007/PL00006487 |author=Pesole G, Gissi C, De Chirico A, Saccone C |title=Nucleotide substitution rate of mammalian mitochondrial genomes |journal=J. Mol. Evol. |volume=48 |issue=4 |pages=427–34 |date=April 1999 |pmid=10079281}}</ref><ref>Huang, S. (2008) The genetic equidistance result of molecular evolution is independent of mutation rates. J. Comp. Sci. Syst. Biol., 1: 92-102. http://omicsonline.com/ArchiveJCSB/Ab01/JCSB1.092.html</ref>
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| Later [[Allan Wilson]] and [[Vincent Sarich]] built upon this work.
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| ==Relationship with neutral theory==
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| The observation of a clock-like rate of molecular change was originally purely [[Phenomenology (science)|phenomenological]]. Later, the work of [[Motoo Kimura]]<ref name=Kimura68>{{cite journal |last=Kimura |first=Motoo |year=1968 |title= Evolutionary rate at the molecular level |journal= Nature |volume=217 |pages=624–626 |doi=10.1038/217624a0 |pmid=5637732 |issue=5129}}</ref> developed the [[neutral theory of molecular evolution]], which predicted a molecular clock. Let there be N individuals, and to keep this calculation simple, let the individuals be [[Ploidy|haploid]] (i.e. have one copy of each gene). Let the rate of neutral [[mutation]]s (i.e. mutations with no effect on [[Fitness (biology)|fitness]]) in a new individual be <math>\mu</math>. The probability that this new mutation will become [[Fixation (population genetics)|fixed]] in the population is then 1/N, since each copy of the gene is as good as any other. Every generation, each individual can have new mutations, so there are <math>\mu</math>N new neutral mutations in the population as a whole. That means that each generation, <math>\mu</math> new neutral mutations will become fixed. If most changes seen during [[molecular evolution]] are neutral, then [[Fixation (population genetics)|fixations]] in a population will accumulate at a clock-rate that is equal to the rate of neutral [[mutation]]s in an individual.
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| ==Calibration==
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| The molecular clock alone can only say that one time period is twice as long as another: it cannot assign concrete dates. To achieve this, the molecular clock must first be [[calibration|calibrated]] against independent evidence about dates, such as the [[fossil]] record.<ref name=Benton01>{{cite journal |author=benton, M. J. and Donoghue, P. C. J. |year=200t |title= Paleontological evidence to date the Tree of Life|journal=Molecular Biology & Evolution|volume= 24|pages=26–53 |doi=10.1093/molbev/msl150 |pmid=17047029 |issue=1}}</ref> Alternatively, for viral phylogenetics and [[ancient DNA]] studies, two areas of evolutionary biology where it is possible to sample sequences over an evolutionary timescale, the dates of the samples themselves can be used to calibrate the molecular clock.
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| ==Non-constant rate of molecular clock==
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| Sometimes only a single divergence date can be estimated from fossils, with all other dates inferred from that. Other sets of species have abundant fossils available, allowing the MCH of constant divergence rates to be tested. DNA sequences experiencing low levels of [[negative selection (natural selection)|negative selection]] showed divergence rates of 0.7-0.8% per [[Myr]] in bacteria, mammals, invertebrates, and plants.<ref name=Ochman87>{{cite journal |author=Ochman H, Wilson AC. |journal=J Mol Evol.| year=1987| pages=74–86.|title=Evolution in bacteria: evidence for a universal substitution rate in cellular genomes | volume=26|doi=10.1007/BF02111283 |pmid=3125340 |issue=1–2}}</ref> In the same study, genomic regions experiencing very high negative or purifying selection (encoding rRNA) were considerably slower (1% per 50 Myr).
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| In addition to such variation in rate with genomic position, since the early 1990s, variation among taxa has proven fertile ground for research too,<ref name=Douzery03>{{cite journal |author=Douzery, E.J.P., Delsuc, F., Stanhope, M.J. and Huchon, D. |year=2003 |title= Local molecular clocks in three nuclear genes: divergence times for rodents and other mammals, and incompatibility among fossil calibrations|journal=Journal of Molecular Evolution |volume= 57|pages=S201–S213|url= |doi=10.1007/s00239-003-0028-x |pmid=15008417}}</ref> even over comparatively short periods of evolutionary time (for example [[mockingbird]]s<ref name=Hunt01>{{cite journal |author=Hunt, J.S., Bermingham, E., and Ricklefs, R.E. |year=2001 |title=Molecular systematics and biogeography of Antillean thrashers, tremblers, and mockingbirds (Aves: Mimidae) |journal=[[Auk (journal)|Auk]] |volume=118 |issue=1 |pages=35–55|url=http://findarticles.com/p/articles/mi_qa3793/is_200101/ai_n8930531 |doi=10.1642/0004-8038(2001)118[0035:MSABOA]2.0.CO;2 |issn=0004-8038 }}</ref>). [[Procellariiformes|Tube-nosed seabirds]] have molecular clocks that on average run at half speed of many other birds,<ref name=Rheindt05>{{cite journal |author=Rheindt, F. E. and Austin, J. |year=2005 |title=Major analytical and conceptual shortcomings in a recent taxonomic revision of the Procellariiformes - A reply to Penhallurick and Wink (2004) |journal=[[Emu (journal)|Emu]] |volume=105 |issue=2 |pages=181–186|url=http://www.publish.csiro.au/?act=view_file&file_id=MU04039.pdf |doi=10.1071/MU04039 }}</ref> possibly due to long [[generation]] times, and many turtles have a molecular clock running at one-eighth the speed it does in small mammals or even slower.<ref name=Avise92>{{cite journal |author= Avise, J.C., Bowen, W., Lamb, T., Meylan, A.B. and Bermingham, E. |date=1 May 1992|title= Mitochondrial DNA Evolution at a Turtle's Pace: Evidence for Low Genetic Variability and Reduced Microevolutionary Rate in the Testudines |journal= Molecular Biology and Evolution |volume= 9|issue= 3|pages=457–473|url= http://mbe.oxfordjournals.org/cgi/reprint/9/3/457 |pmid= 1584014 }}</ref> Effects of [[small population size]] are also likely to confound molecular clock analyses; [[cheetah]]s for example, having gone through at least 2 [[population bottleneck]]s, could not be adequately studied based on a molecular clock model alone.{{Citation needed|date=January 2008}} Researchers such as Francisco Ayala have more fundamentally challenged the molecular clock hypothesis.<ref name=Ayala99>{{cite journal |author= Ayala, F.J.|year=1999 |title=Molecular clock mirages |journal=[[BioEssays]] |volume=21|issue=1 |pages=71–75|url=http://www3.interscience.wiley.com/cgi-bin/abstract/60000186/ABSTRACT?CRETRY=1&SRETRY=0 |doi=10.1002/(SICI)1521-1878(199901)21:1<71::AID-BIES9>3.0.CO;2-B |pmid=10070256}}</ref><ref name=Schwartz06>{{cite journal |author=Schwartz, J. H. and Maresca, B. |year=2006 |title=Do Molecular Clocks Run at All? A Critique of Molecular Systematics |journal=Biological Theory |volume=1 |pages=357–371|url= |doi=10.1162/biot.2006.1.4.357|laysummary=http://www.sciencedaily.com/releases/2007/02/070210170623.htm|laysource=[[Science Daily]] |issue=4}}</ref> According to Ayala's 1999 study, 5 factors combine to limit the application of molecular clock models:
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| * Changing generation times (If the rate of new mutations depends at least partly on the number of generations rather than the number of years)
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| * Population size ([[Genetic drift]] is stronger in small populations, and so more mutations are effectively neutral)
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| * Species-specific differences (due to differing metabolism, ecology, evolutionary history,...)
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| * Change in function of the protein studied (can be avoided in closely related species by utilizing [[non-coding DNA]] sequences or emphasizing [[silent mutation]]s)
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| * Changes in the intensity of natural selection.
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| Molecular clock users have developed workaround solutions using a number of statistical approaches including [[maximum likelihood]] techniques and later [[Bayesian statistics|Bayesian modeling]]. In particular, models that take into account rate variation across lineages have been proposed in order to obtain better estimates of divergence times. These models are called '''relaxed molecular clocks'''<ref name=Drummond06>{{cite journal |author=Drummond, A.J., Ho, S.Y.W., Phillips, M.J. and Rambaut A. |year=2006 |title= Relaxed Phylogenetics and Dating with Confidence|journal= [[Public Library of Science|PLoS Biology]] |volume= 4|issue=5 |pages=e88|doi= 10.1371/journal.pbio.0040088 |pmid=16683862 |pmc=1395354}}</ref> because they represent an intermediate position between the 'strict' molecular clock hypothesis and Felsenstein's many-rates model and are made possible through [[Markov chain Monte Carlo|MCMC]] techniques that explore a weighted range of tree topologies and simultaneously estimate parameters of the chosen substitution model. It must be remembered that divergence dates inferred using a molecular clock are based on statistical [[inference]] and not on direct [[evidence]].
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| The molecular clock runs into particular challenges at very short and very long timescales. At long timescales, the problem is [[Saturation (genetic)|saturation]]. When enough time has passed, many sites have undergone more than one change, but it is impossible to detect more than one. This means that the observed number of changes is no longer [[Linear function|linear]] with time, but instead flattens out.
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| At very short time scales, many differences between samples do not represent [[Fixation (population genetics)|fixation]] of different sequences in the different populations. Instead, they represent alternative [[alleles]] that were both present as part of a polymorphism in the common ancestor. The inclusion of differences that have not yet become [[Fixation (population genetics)|fixed]]
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| leads to a potentially dramatic inflation of the apparent rate of the molecular clock at very short timescales.<ref>{{cite journal |doi=10.1093/molbev/msi145 |author=Ho SYW, Phillips MJ, Cooper A, Drummond AJ |year=2005 |title= Time dependency of molecular rate estimates and systematic overestimation of recent divergence times |journal= Molecular Biology & Evolution |volume= 22 |issue=7 |pages=1561–1568 |pmid=15814826}}</ref><ref>{{cite journal |author=Peterson GI, Masel J| year=2009 |title= Quantitative Prediction of Molecular Clock and Ka/Ks at Short Timescales |journal= Molecular Biology & Evolution |pmid=19661199 |volume= 26 |issue=11 |pmc=2912466 |pages=2595–2603| doi=10.1093/molbev/msp175}}</ref>
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| == Methods ==
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| Bayesian methods can provide more appropriate estimates of divergence times, especially if large datasets - such as those yielded by [[phylogenomics]] - are employed.<ref name="Dos Reis2012">{{cite doi|10.1098/rspb.2012.0683}}</ref>
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| ==Uses==
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| The molecular clock technique is an important tool in [[molecular systematics]], the use of [[molecular genetics]] information to determine the correct [[scientific classification]] of organisms or to study variation in selective forces.
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| Knowledge of approximately constant rate of molecular evolution in particular sets of lineages also facilitates establishing the dates of [[phylogeny|phylogenetic]] events, including those not documented by [[fossils]], such as the divergence of living [[taxon|taxa]] and the formation of the [[phylogenetic tree]]. But in these cases — especially over long stretches of time — the limitations of MCH (above) must be considered; <!-- There is a critique in TREE journal that puts a good point on this --> such estimates may be off by 50% or more.
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| ==See also==
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| * [[Gene orders]]
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| * [[Human mitochondrial molecular clock]]
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| * [[Mitochondrial Eve]] and [[Y-chromosomal Adam]]
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| * [[Models of DNA evolution]]
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| * [[Molecular evolution]]
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| * [[Neutral theory of molecular evolution]]
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| ==References==<!-- ZoolScripta35:531 molecular evolution speeds can differ markedly after few million years already -->
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| {{Reflist|2}}
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| ==Further reading==
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| * {{Cite journal |author=Morgan, G.J. |year=1998 |title=Emile Zuckerkandl, Linus Pauling, and the Molecular Evolutionary Clock, 1959-1965 |journal=Journal of the History of Biology |volume=31 |issue=2 |pages=155–178 |doi=10.1023/A:1004394418084|url=http://www.springerlink.com/content/l1723tq0q2751215/fulltext.pdf |pmid=11620303}}
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| * {{Cite book | author = [[Emile Zuckerkandl|Zuckerkandl, E.]] and [[Linus Pauling|Pauling, L.B.]] | year = 1965 | title = Evolving Genes and Proteins | chapter = Evolutionary divergence and convergence in proteins | editor = Bryson, V.and Vogel, H.J. (editors) | pages = 97–166 | publisher = Academic Press, New York}}
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| *{{cite journal
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| | last = San Mauro
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| | first = D.
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| | title = Molecular systematics: a synthesis of the common methods and the state of knowledge
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| | journal = Cellular & Molecular Biology Letters
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| | volume = 15
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| | pages = 311–341
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| | year = 2010
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| | coauthors = Agorreta, A.
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| | doi=10.2478/s11658-010-0010-8
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| | issue = 2
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| }}
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| == External links ==
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| * [http://web.archive.org/web/20100525101859/http://awcmee.massey.ac.nz/aw.htm Allan Wilson and the molecular clock]
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| * [http://web.archive.org/web/20090213150149/http://rtis.com/nat/user/elsberry/evobio/evc/argresp/sequence.html Molecular clock explanation of the molecular equidistance phenomenon]
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| * [http://www.fossilrecord.net/dateaclade/index.html Date-a-Clade service for the molecular tree of life]
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| {{Chronology}}
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| {{Use dmy dates|date=August 2010}}
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| {{DEFAULTSORT:Molecular Clock}}
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| [[Category:Molecular evolution]]
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| [[Category:Molecular genetics]]
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| [[Category:Phylogenetics]]
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However this isn't completely surprising, because a big waist (excess belly fat) is tied carefully to sort 2 diabetes, insulin resistance, a bad cholesterol profile plus heart condition. Excess fat in the belly indicates excess fat surrounding the abdominal organs -- never superior news; this form of fat is risky.
If left unchecked, plaque may build up in the arteries to the heart or mind leading to a heart attack or stroke. It's wise to keep an eye on your numbers.
Number 7. Test plus check a equipment before the marriage day. And by equipment, it literally means everything. From the camera, to the speed lights, a laptop, the reflectors plus naturally all of the batteries, check and test everything. Know which bulbs plus batteries have to be changed. There is really much space for error plus wedding photographers dont need their trusted equipment to fail on W-day, which might be a nightmare.
Since strokes are so dangerous, it's important to work on reducing the dangers that are under the control. Keep the blood pressure in a healthy range, as this is among the right elements you are able to do to fight the risk.
To discover out more, a research team searched health literature for research which looked at stroke risk and body mass index with a minimal of four years of follow up.
Underwater Weighing - Underwater weighing, or hydrostatic weighing, is another system to determine the body fat . In this procedure, a individual sits inside a specialized seat, expels the air from their lungs, and waist to height ratio is then submerged into a big steel tank of water to stay motionless while their underwater weight is recorded. This is repeated until a dependable fat is measured. The purpose is to measure density of the body plus calculate yourfat fat. Because lean tissue is denser than fat tissue, this system may determine the buoyancy of the person whilst underwater. Once considered the gold standard offat measurement, the equipment is quite costly, creating alternative techniques more appealing.
The techniques provided inside the program are based on the truth that there is not any fast means to burn excess body fat, however, it may nevertheless be possible by implementing the protocols, like following a diet plan with necessary exercises. The diet includes a list of food items including fruits that are capable to burn body fat immediately.
All info on this website is for informational reasons only. It is not intended to diagnose, treat, cure, or avoid any wellness problem - nor is it intended to replace the advice of a qualified healthcare specialist, health specialist, or fitness expert. No action could be taken only on the contents of this website. Always consult the doctor or qualified health specialist on any matters regarding your wellness or on any opinions expressed inside this site.