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{{Refimprove|date=January 2014}}[[Image:Dichloromethane near IR spectrum.png|thumb|right|450px|Near IR absorption spectrum of [[dichloromethane]] showing complicated overlapping [[overtones]] of mid IR absorption features.]]
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'''Near-infrared spectroscopy''' (NIRS) is a [[spectroscopic]] method that uses the near-[[infrared]] region of the [[electromagnetic spectrum]] (from about 800 nm to 2500 nm). Typical applications include [[pharmaceutical]], medical diagnostics (including [[blood sugar]] and [[pulse oximetry]]), food and agrochemical quality control, and combustion research, as well as research in [[functional neuroimaging]], sports medicine & science, elite sports training, [[ergonomics]], [[Physical therapy|rehabilitation]],  [[neonatal]] research, [[brain computer interface]], [[urology]] (bladder contraction) and [[neurology]] (neurovascular coupling).


==Theory==
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[[Near-infrared]] spectroscopy is based on molecular overtone and combination vibrations. Such transitions are [[forbidden transition|forbidden]] by the [[selection rule]]s of [[quantum mechanics]]. As a result, the [[molar absorptivity]] in the near IR region is typically quite small. One advantage is that NIR can typically penetrate much farther into a sample than [[infrared spectroscopy|mid infrared]] radiation. Near-infrared spectroscopy is, therefore, not a particularly sensitive technique, but it can be very useful in probing bulk material with little or no sample preparation.
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The molecular overtone and combination bands seen in the near IR are typically very broad, leading to complex spectra; it can be difficult to assign specific features to specific chemical components. [[Multivariate statistics|Multivariate]] (multiple variables) calibration techniques (e.g., [[principal components analysis]], [[partial least squares]], or [[artificial neural networks]]) are often employed to extract the desired chemical information. Careful development of a set of calibration samples and application of multivariate calibration techniques is essential for near-infrared analytical methods.<ref name=Balabin_2007>{{cite journal |journal=[[Chemometrics and intelligent laboratory systems|Chemometr Intell Lab]] |volume = 88 |issue = 2 |pages = 183–188 |doi=10.1016/j.chemolab.2007.04.006 |title=Comparison of linear and nonlinear calibration models based on near infrared (NIR) spectroscopy data for gasoline properties prediction |year=2007 |author=Roman M. Balabin, Ravilya Z. Safieva, and Ekaterina I. Lomakina}}</ref>
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==History==
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[[Image:Ethanol near IR spectrum.png|thumb|right|250px|[[Near infrared spectrum]] of liquid ethanol.]]
The discovery of near-infrared energy is ascribed to [[William Herschel]] in the 19th century, but the first industrial application began in the 1950s. In the first applications, NIRS was used only as an add-on unit to other optical devices that used other wavelengths such as [[ultraviolet]] (UV), visible (Vis), or mid-infrared (MIR) spectrometers. In the 1980s, a single unit, stand-alone NIRS system was made available, but the application of NIRS was focused more on chemical analysis. With the introduction of light-[[fiber optics]] in the mid-1980s and the monochromator-detector developments in early-1990s, NIRS became a more powerful tool for scientific research.


This optical method can be used in a number of fields of science including [[physics]], [[physiology]], or medicine. It is only in the last few decades that NIRS began to be used as a medical tool for monitoring patients.
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==Instrumentation==
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Instrumentation for near-IR (NIR) spectroscopy is similar to instruments for the UV-visible and mid-IR ranges. There is a source, a detector, and a dispersive element (such as a [[prism (optics)|prism]], or, more commonly, a [[diffraction grating]]) to allow the intensity at different wavelengths to be recorded. [[Fourier transform spectroscopy|Fourier transform NIR instruments]] using an [[interferometer]] are also common, especially for wavelengths above ~1000&nbsp;nm. Depending on the sample, the spectrum can be measured in either reflection or transmission.
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Common [[incandescent]] or quartz halogen light bulbs are most often used as broadband sources of near-infrared radiation for analytical applications. [[Light-emitting diode]]s (LEDs) are also used; they offer greater lifetime and spectral stability and reduced power requirements.<ref>{{cite journal|doi=10.1186/1475-925X-4-29|year=2005|last1=Bozkurt|first1=Alper|last2=Rosen|first2=Arye|last3=Rosen|first3=Harel|last4=Onaral|first4=Banu|journal=BioMedical Engineering OnLine|volume=4|pages=29|pmid=15862131|title=A portable near infrared spectroscopy system for bedside monitoring of newborn brain.|issue=1|pmc=1112605}}</ref>
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The type of detector used depends primarily on the range of wavelengths to be measured. Silicon-based [[charge-coupled device|CCD]]s are suitable for the shorter end of the NIR range, but are not sufficiently sensitive over most of the range (over 1000&nbsp;nm). [[InGaAs]] and [[Lead(II) sulfide|PbS]] devices are more suitable though less sensitive than CCDs. In certain diode array (DA) NIRS instruments, both silicon-based and InGaAs detectors are employed in the same instrument. Such instruments can record both UV-visible and NIR spectra 'simultaneously'.
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Instruments intended for [[chemical imaging]] in the NIR may use a 2D array detector with an [[acousto-optic]] tunable filter. Multiple images may be recorded sequentially at different narrow wavelength bands.<ref>{{cite journal|title=Near-Infrared Acousto-Optic Filtered Spectroscopic Microscopy: A Solid-State Approach to Chemical Imaging|journal=Applied Spectroscopy|year = 1992|volume = 46|pages = 553–559|author = Treado, P. J.; Levin, I. W.; Lewis, E. N.|doi = 10.1366/0003702924125032|bibcode = 1992ApSpe..46..553T }}</ref>
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Many commercial instruments for UV/vis spectroscopy are capable of recording spectra in the NIR range (to perhaps ~900&nbsp;nm). In the same way, the range of some mid-IR instruments may extend into the NIR. In these instruments, the detector used for the NIR wavelengths is often the same detector used for the instrument's "main" range of interest.
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==Applications==
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Typical applications of NIR spectroscopy include the analysis of foodstuffs, pharmaceuticals, combustion products and a major branch of astronomical spectroscopy.
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===Astronomical spectroscopy===
</ul>
 
Near-infrared [[spectroscopy]] is used in [[astronomy]] for studying the atmospheres of cool stars where molecules can form. The vibrational and rotational signatures of molecules such as titanium oxide, cyanide, and carbon monoxide can be seen in this [[wavelength]] range and can give a clue towards the star's [[spectral type]]. It is also used for studying molecules in other astronomical contexts, such as in [[molecular clouds]] where new stars are formed. The astronomical phenomenon known as [[interstellar reddening|reddening]] means that near-infrared wavelengths are less affected by dust in the interstellar medium, such that regions inaccessible by optical spectroscopy can be studied in the near-infrared. Since dust and gas are strongly associated, these dusty regions are exactly those where infrared spectroscopy is most useful. The near-infrared spectra of very young stars provide important information about their ages and masses, which is important for understanding star formation in general.
 
===Agriculture===
Near-infrared [[spectroscopy]] is widely applied in agriculture for determining the quality of forages, grains, and grain products, oilseeds, coffee, tea, spices, fruits, vegetables, sugarcane, beverages, fats, and oils, dairy products, eggs, meat, and other agricultural products. It is widely used to quantify the composition of agricultural products because it meets the criteria of being accurate, reliable, rapid, non-destructive, and inexpensive.<ref>{{cite isbn|9780849373930|pages=349-369}}</ref>
 
===Remote monitoring===
 
Techniques have been developed for NIR spectroscopic imaging. [[Hyperspectral imaging]] has been applied for a wide range of uses, including the remote investigation of plants and soils. Data can be collected from instruments on airplanes or satellites to assess ground cover and soil chemistry.
 
===Materials Science===
 
Techniques have been developed for NIR spectroscopy of microscopic sample areas for film thickness measurements, research into the optical characteristics of nanoparticles and optical coatings for the telecommunications industry.
 
===Medical uses===
 
The primary application of NIRS to the human body uses the fact that the transmission and absorption of NIR light in human body tissues contains information about hemoglobin concentration changes. When a specific area of the brain is activated, the localized blood volume in that area changes quickly. Optical imaging can measure the location and activity of specific regions of the brain by continuously monitoring blood hemoglobin levels through the determination of optical absorption coefficients.
 
NIRS can be used for non-invasive assessment of brain function through the intact skull in human subjects by detecting changes in blood hemoglobin concentrations associated with neural activity, e.g., in branches of [[Cognitive psychology]] as a partial replacement for [[fMRI]] techniques.<ref>{{cite journal|year=2002|last1=Mehagnoul-Schipper|first1=DJ|last2=van der Kallen|first2=BF|last3=Colier|first3=WNJM|last4=van der Sluijs|first4=MC|last5=van Erning|first5=LJ|last6=Thijssen|first6=HO|last7=Oeseburg|first7=B|last8=Hoefnagels|first8=WH|last9=Jansen|first9=RW|title=Simultaneous measurements of cerebral oxygenation changes during brain activation by near-infrared spectroscopy and functional magnetic resonance imaging in healthy young and elderly subjects. |url = http://spin.ecn.purdue.edu/fmri/PDFLibrary/MehagnoulSchipperDJ_HBM_2002_16_14_23.pdf | doi = 10.1002/hbm.10026|volume=16|issue=1|pages=14–23|journal=Hum Brain Mapp|pmid=11870923}}</ref> NIRS can be used on infants, and NIRS is much more portable than fMRI machines, even wireless instrumentation is available, which enables investigations in freely moving subjects.<ref>{{cite journal|doi=10.1364/OE.16.010323|pmid=18607442|url=http://www.neonatologie.usz.ch/Documents/LehreUndForschung/Publikationen/Muehlemann%20Wireless%20miniaturized.pdf|year=2008|last1=Muehlemann|first1=T|last2=Haensse|first2=D|last3=Wolf|first3=M|title=Wireless miniaturized in-vivo near infrared imaging.|volume=16|issue=14|pages=10323–30|journal=Optics express|bibcode = 2008OExpr..1610323M }}</ref><ref>{{cite journal|doi=10.3233/SPE-2009-0391|url=http://iospress.metapress.com/content/x482652580863660|year=2009|last1=Shadgan|first1=B|last2=Reid|first2=W|last3=Gharakhanlou|first3=R|last4=Stothers|first4=L|last5=Macnab|first5=A| title=Wireless near-infrared spectroscopy of skeletal muscle oxygenation and hemodynamics during exercise and ischemia.|volume=23|issue=5-6|pages=233–241|journal=Spectroscopy}}</ref> However, NIRS cannot fully replace fMRI because it can only be used to scan cortical tissue, where fMRI can be used to measure activation throughout the brain. Special public domain statistical toolboxes for analysis of stand alone and combined NIRS/MRI measurement have been developed<ref>{{citation |url=http://bisp.kaist.ac.kr/papers/Ye09_NeuroImage.pdf|year=2009|last1=Ye |first1=JC|last2=Tak |first3=S|last2=Jan |first3=KE|last4=Jung |first4=J|last5=Jang |first5=J|title=NIRS-SPM: statistical parametric mapping for near-infrared spectroscopy.|volume=44|issue=2|pages=428–47|journal=Neuroimage}}</ref> ([http://bisp.kaist.ac.kr/NIRS-SPM.html NIRS-SPM]).
 
The application in functional mapping of the human cortex is called diffuse optical tomography (DOT), near infrared imaging (NIRI) or functional NIRS ([[fNIR]]). The term diffuse optical tomography is used for three-dimensional NIRS. The terms NIRS, NIRI and DOT are often used interchangeably, but they have some distinctions. The most important difference between NIRS and DOT/NIRI is that DOT/NIRI is used mainly to detect changes in optical properties of tissue simultaneously from multiple measurement points and display the results in the form of a map or image over a specific area, whereas NIRS provides quantitative data in absolute terms on up to a few specific points. The latter is also used to investigate other tissues such as, e.g., muscle,<ref name="dare.ubn.kun.nl">{{citation |url=http://dare.ubn.kun.nl/bitstream/2066/19136/1/19136_quannespi.pdf|year=2002|last1=van Beekvelt |first1=MCP| title=Quantitative near-infrared spectroscopy in human skeletal muscle methodological issues and clinical application.| journal=PhD thesis, University of Nijmegen }}</ref> breast and tumors.<ref>{{citation |year=1999|last1=Van der Sanden |first1=BP|last2=Heerschap |first2=A |last3=Hoofd |first3=L |last4=Simonetti |first4=AW |last5=Nicolay |first5=K |last6=van der Toorn |first6=A |last7=Colier |first7=WNJM |last8=van der Kogel |first8= AJ
title=Effect of carbogen breathing on the physiological profile of human glioma xenografts| journal=Magn Reson Me|volume=42|issue=3|pages=490–9 |pmid=10467293}}</ref>  NIRS can be used to quantify blood flow, blood volume, oxygen consumption, reoxygenation rates and muscle recovery time in muscle.<ref name="dare.ubn.kun.nl"/>
 
By employing several wavelengths and time resolved (frequency or time domain) and/or spatially resolved methods blood flow, volume and absolute tissue saturation (<math>StO_2</math> or Tissue Saturation Index (TSI)) can be quantified.<ref name="ReferenceA">{{cite journal|pmid=18163807|url=http://www.neonatologie.usz.ch/Documents/LehreUndForschung/Publikationen/Wolf2007JBOReview.pdf|year=2007|last1=Wolf|first1=M|last2=Ferrari|first2=M|last3=Quaresima|first3=V|title=Progress of near-infrared spectroscopy and topography for brain and muscle clinical applications.|volume=12|issue=6|pages=062104|doi=10.1117/1.2804899|journal=Journal of biomedical optics|bibcode = 2007JBO....12f2104W }}</ref> Applications of oximetry by NIRS methods include neuroscience, ergonomics, rehabilitation, brain computer interface, urology, the detection of illnesses which affect the blood circulation (e.g., peripheral vascular disease), the detection and assessment of breast tumors, and the optimization of training in sports medicine. 
 
The use of NIRS in conjunction with a bolus injection of [[indocyanine green]] (ICG) has been used to measure cerebral blood flow<ref>{{citation |pmid=14568455|year=2003|last1=Keller |first1=E|last2=Nadler |first2=A|last3=Alkadhi |first3=H|last4=Kollias |first4=SS|last5=Yonekawa |first5=Y|last6=Niederer |first6=P|title=Noninvasive measurement of regional cerebral blood flow and regional cerebral blood volume by near-infrared spectroscopy and indocyanine greene dye dilution.|volume=20|pages=828–839|journal=Neuroimage }}</ref><ref>{{cite journal|doi=10.1203/00006450-200205000-00004|pmid=11978878|year=2002|last1=Brown|first1=DW|last2=Picot|first2=PA|last3=Naeini|first3=JG|last4=Springett|first4=R|last5=Delpy|first5=DT|last6=Lee|first6=TY|title=Quantitative near infrared spectroscopy measurement of cerebral hemodynamics in newborn piglets.|volume=51|issue=5|pages=564–70|journal=Pediatric research}}</ref> and cerebral metabolic rate of oxygen consumption.<ref>{{cite journal|pmid=16192991|year=2006|last1=Tichauer|first1=KM|last2=Hadway|first2=JA|last3=Lee|first3=TY|last4=St Lawrence|first4=K|title=Measurement of cerebral oxidative metabolism with near-infrared spectroscopy: a validation study.|volume=26|issue=5|pages=722–30|doi=10.1038/sj.jcbfm.9600230|journal=Journal of Cerebral Blood Flow and Metabolism}}</ref>
It has also been shown that CMRO2 can be calculated with combined NIRS/MRI measurements.<ref>{{citation |pmid=20479515|year=2010|last1=Tak |first1=S|last2=Jang |first2=J|last3=Lee |first3=K|last4=Ye |first4=JC|title=Quantification of CMRO(2) without hypercapnia using simultaneous near-infrared spectroscopy and fMRI measurements.|volume=55|issue=11|pages=3249–69|journal=Phys Med Biol}}</ref>
 
NIRS is starting to be used in pediatric critical care, to help deal with cardiac surgery post-op. Indeed, NIRS is able to measure venous oxygen saturation (SVO2), which is determined by the cardiac output, as well as other parameters (FiO2, hemoglobin, oxygen uptake). Therefore, following the NIRS gives critical care physicians a notion of the cardiac output. NIRS is liked by patients, because it is non-invasive, is painless, and uses non-ionizing radiation.
 
[[Optical Coherence Tomography]] (OCT) is another NIR medical imaging technique capable of 3D imaging with high resolution on par with low-power microscopy. Using optical coherence to measure photon pathlength allows OCT to build images of live tissue and clear examinations of tissue morphology. Due to technique differences OCT is limited to imaging 1–2&nbsp;mm below tissue surfaces, but despite this limitation OCT has become an established [[medical imaging]] technique especially for imaging of the [[retina]] and anterior segments of the eye.
 
The instrumental development of NIRS/NIRI/DOT/OCT has proceeded tremendously during the last years and, in particular, in terms of quantification, imaging and miniaturization.<ref name="ReferenceA"/>
 
===Particle measurement===
 
NIR is often used in particle sizing in a range of different fields, including studying pharmaceutical and agricultural powders.
 
===Industrial uses===
As opposed to NIRS used in optical topography, general NIRS used in chemical assays does not provide imaging by mapping. For example, a clinical [[carbon dioxide]] analyzer requires reference techniques and calibration routines to be able to get accurate CO<sub>2</sub> content change. In this case, calibration is performed by adjusting the zero control of the sample being tested after purposefully supplying 0% CO<sub>2</sub> or another known amount of CO<sub>2</sub> in the sample. Normal compressed gas from distributors contains about 95% O<sub>2</sub> and 5% CO<sub>2</sub>, which can also be used to adjust %CO<sub>2</sub> meter reading to be exactly 5% at initial calibration.{{Citation needed|date=February 2007}}
 
==See also==
{{colbegin|3}}
*[[Chemical Imaging]]
*[[Hyperspectral imaging]]
*[[Functional near-infrared spectroscopy|Functional near-infrared spectroscopy (fNIR)]]
*[[Fourier transform spectroscopy]]
*[[Infrared spectroscopy]]
*[[Optical imaging]]
*[[Rotational spectroscopy]]
*[[Spectroscopy]]
*[[Vibrational spectroscopy]]
{{colend}}
 
==References==
{{Reflist|2}}
 
==Further reading==
*Kouli, M.: "Experimental investigations of non invasive measuring of cerebral blood flow in adult human using the near infrared spectroscopy." Dissertation, [[Technical University of Munich]], December 2001.
*Raghavachari, R., Editor. 2001.'' Near-Infrared Applications in Biotechnology'', Marcel-Dekker, New York, NY.
*Workman, J.; Weyer, L. 2007. ''Practical Guide to Interpretive Near-Infrared Spectroscopy'', CRC Press-Taylor & Francis Group, Boca Raton, FL.
 
{{DEFAULTSORT:Near Infrared Spectroscopy}}
[[Category:Spectroscopy]]
[[Category:Infrared technology]]

Latest revision as of 16:59, 4 January 2015

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