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{{Infobox enzyme
| Name = Isoleucine N-monooxygenase
| EC_number = 1.14.13.117
| CAS_number = 
| IUBMB_EC_number = 1/14/13/117
| GO_code = 
| image = 
| width = 
| caption =
}}
'''Isoleucine N-monooxygenase''' ({{EC number|1.14.13.117}}, ''CYP79D3'', ''CYP79D4'') is an [[enzyme]] with system name ''L-isoleucine,NADPH:oxygen oxidoreductase (N-hydroxylating)''.<ref>{{cite journal | title = Cytochromes ''P''-450 from cassava (''Manihot esculenta'' Crantz) catalyzing the first steps in the biosynthesis of the cyanogenic glucosides linamarin and  lotaustralin. Cloning, functional expression in ''Pichia pastoris'', and  substrate specificity of the isolated recombinant enzymes |author = Andersen, M.D., Busk, P.K., Svendsen, I. and Moller, B.L. |journal = J. Biol. Chem. |year = 2000 |volume = 275 |pages = 1966–1975 |pmid = 10636899 |issue=3}}</ref><ref>{{cite journal | title = Biosynthesis of the nitrile glucosides rhodiocyanoside A and D and the cyanogenic glucosides lotaustralin and linamarin in ''Lotus japonicus'' |author = Forslund, K., Morant, M., Jorgensen, B., Olsen, C.E., Asamizu, E., Sato, S., Tabata, S. and Bak, S. |journal = Plant Physiol. |year = 2004 |volume = 135 |pages = 71–84 |pmid = 15122013 |doi=10.1104/pp.103.038059 |issue=1 |pmc=429334}}</ref> This enzyme [[catalysis|catalyses]] the following [[chemical reaction]]

: L-isoleucine + 2 O<sub>2</sub> + 2 NADPH + 2 H<sup>+</sup> <math>\rightleftharpoons</math> (E)-2-methylbutanal oxime + 2 NADP<sup>+</sup> + CO<sub>2</sub> + 3 H<sub>2</sub>O (overall reaction)
:(1a) L-isoleucine + O<sub>2</sub> + NADPH + H<sup>+</sup> <math>\rightleftharpoons</math> N-hydroxy-L-isoleucine + NADP<sup>+</sup> + H<sub>2</sub>O
:(1b) N-hydroxy-L-isoleucine + O<sub>2</sub> + NADPH + H+ <math>\rightleftharpoons</math> N,N-dihydroxy-L-isoleucine + NADP<sup>+</sup> + H<sub>2</sub>O
:(1c) N,N-dihydroxy-L-isoleucine <math>\rightleftharpoons</math> (E)-2-methylbutanal oxime + CO<sub>2</sub> + H<sub>2</sub>O (spontaneous reaction)

Isoleucine N-monooxygenase is a [[heme]]-[[thiol]]ate protein (P-450).

== References ==
{{reflist}}

== External links ==
* {{MeshName|Isoleucine+N-monooxygenase}}

[[Category:EC 1.14.13]]