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{{Infobox enzyme
| Name = TRNA pseudouridine<sup>32</sup> synthase
| EC_number = 5.4.99.28
| CAS_number = 430429-15-5
| IUBMB_EC_number = 5/4/99/28
| GO_code = 
| image = 
| width = 
| caption =
}}
'''TRNA pseudouridine<sup>32</sup> synthase''' ({{EC number|5.4.99.28}}, ''RluA'', ''pseudouridine synthase RluA'', ''Pus9p'', ''Rib2/Pus8p'') is an [[enzyme]] with system name ''tRNA-uridine<sup>32</sup> uracil mutase''.<ref>{{cite journal | title = Crystal structure of pseudouridine synthase RluA: indirect sequence readout through protein-induced RNA structure |author = Hoang, C., Chen, J., Vizthum, C.A., Kandel, J.M., Hamilton, C.S., Mueller, E.G. and Ferre-D'Amare, A.R. |journal = Mol. Cell |year = 2006 |volume = 24 |pages = 535–545 |pmid = 17188032}}</ref><ref>{{cite journal | title = Functional importance of motif I of pseudouridine synthases: mutagenesis of aligned lysine and proline residues |author = Spedaliere, C.J., Hamilton, C.S. and Mueller, E.G. |journal = Biochemistry |year = 2000 |volume = 39 |pages = 9459–9465 |pmid = 10924141}}</ref><ref>{{cite journal | title = Functional effect of deletion and mutation of the ''Escherichia coli'' ribosomal RNA and tRNA pseudouridine synthase RluA |author = Raychaudhuri, S., Niu, L., Conrad, J., Lane, B.G. and Ofengand, J. |journal = J. Biol. Chem. |year = 1999 |volume = 274 |pages = 18880–18886 |pmid = 10383384}}</ref><ref>{{cite journal | title = Role of cysteine residues in pseudouridine synthases of different families |author = Ramamurthy, V., Swann, S.L., Spedaliere, C.J. and Mueller, E.G. |journal = Biochemistry |year = 1999 |volume = 38 |pages = 13106–13111 |pmid = 10529181}}</ref><ref>{{cite journal | title = A dual-specificity pseudouridine synthase: an ''Escherichia coli'' synthase purified and cloned on the basis of its specificity for &Psi;<sup>746</sup> in 23S RNA is also specific for &Psi;<sup>32</sup> in tRNA<sup>Phe</sup> |author = Wrzesinski, J., Nurse, K., Bakin, A., Lane, B.G. and Ofengand, J. |journal = RNA |year = 1995 |volume = 1 |pages = 437–448 |pmid = 7493321}}</ref><ref>{{cite journal | title = Pseudouridylation at position 32 of mitochondrial and cytoplasmic tRNAs requires two distinct enzymes in ''Saccharomyces cerevisiae'' |author = Behm-Ansmant, I., Grosjean, H., Massenet, S., Motorin, Y. and Branlant, C. |journal = J. Biol. Chem. |year = 2004 |volume = 279 |pages = 52998–53006 |pmid = 15466869}}</ref> This enzyme [[catalysis|catalyses]] the following [[chemical reaction]]

: tRNA uridine<sup>32</sup> <math>\rightleftharpoons</math> tRNA pseudouridine<sup>32</sup>

The dual enzyme from ''[[Escherichia coli]]'' also catalyses the formation of pseudouridine<sup>746</sup> in 23S rRNA.

== References ==
{{reflist}}

== External links ==
* {{MeshName|TRNA+pseudouridine32+synthase}}

[[Category:EC 5.4.99]]